Jul 2026
Designing Fidelity of CRISPR-Cas Endonucleases by Kinetic Insights
A PLM-assisted physics-driven approach is presented that utilizes atomistic molecular dynamics simulations and automated path searching to efficiently obtain the complete kinetic insights, including the transition state structures, for the conformational changes of Cas before DNA cleavage.
Huihui Liu, Zhenyu Zhou, Luowei Yuan et al.
· bioRxiv · 0 citations