Xiu-Chao Zhao

Photo-CIDNP-Enhanced 19F NMR for Rapid Protein Conformation and Ligand Binding Analysis.

19F NMR spectroscopy is a powerful probe of protein conformation and dynamics but is intrinsically limited by low sensitivity common to other NMR techniques. Here, we integrate photochemically induced dynamic nuclear polarization (photo-CIDNP) with genetically encoded 19F labels to enhance sensitivity by up to 220-fold...