Xiu-Mei Mu

A PNA-assisted magnetic bead-confined CbAgo-CRISPR/Cas12a cascade for amplification-free detection of Mycobacterium avium subsp. paratuberculosis F57 DNA.

Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of Johne's disease in ruminants, has attracted increasing attention because of its potential zoonotic relevance. Sensitive molecular detection of MAP typically relies on nucleic acid amplification to overcome low pathogen abundance; however, amplifi...