Construction of Lactococcus lactis Expression System via Chromosomal Integration of T7 RNA Polymerase for Levan Production from Fruit Pomace.
Fruit pomace recycling is critical for green biomanufacturing. Lactococcus lactis, a common host for waste valorization, shows poor protein expression in conventional systems. We constructed T7RNAP-driven inducible and constitutive expression systems in L. lactis NZ9000. The two systems increased target gene transcript...