Xuhui Tian

Recruitment of Cas3 enables DNA cleavage by the type I-A CRISPR-Cas system of Saccharolobus islandicus.

This study isolates the I-A Cascade from Saccharolobus islandicus, revealing a minimal form of Cascade lacking both Cas3 and the CRISPR-RNA maturase Cas6, and shows that ATP not only enables the processive target degradation by Cas3 but also suppresses the trans-cleavage of the same enzyme.