High-Dimensional Multi-Omic Mapping of Post-Mortem Human Brain Using Iterative Indirect Immunofluorescence Imaging on Xenium-Processed Tissues
A post-spatial profiling workflow that combines the Xenium platform with iterative indirect immunofluorescence imaging (4i) on formalin-fixed paraffin-embedded (FFPE) human brain tissue to capture key pathological features for Alzheimer’s disease (AD) and facilitate cell segmentation is reported.