Yasir Ali

Conformational switching of Trp399 regulates opening and closing of loop-1 in galactofuranosyl transferase 2 of Mycobacterium tuberculosis.

Mycobacterial cell-wall assembly depends on multiple glycosyltransferases among which the processive galactofuranosyl transferase 2 (GlfT2) executes alternating β(1 → 5) and β(1 → 6) transfers of galactofuranose (Galf) within a single catalytic site through an oxocarbenium-like transition state. Despite the well charac...