Author

Yasushi Ishihama

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Open access Sep 2026

Proximity Proteomics Maps Candidate Cellular Uptake Pathways for Cell‐Derived Nanovesicles

Although extracellular vesicles (EVs) facilitate selective molecular exchange between cells, their low yields and inherent heterogeneity limit translational applications. Cell‐derived nanovesicles (CDNs), produced by mechanical extrusion of donor cells, offer a scalable alternative while retaining key membrane features...

Eisuke Kanao, Ryosuke Mizuta, Saki Tarao et al. · Small Methods · 0 citations

Open access Sep 2026

Improved Peptide Recovery in Low-Input MS-Based Proteomics Using High-Recovery StageTip Desalting and Nonionic Detergent-Assisted Reconstitution.

Low-input mass spectrometry (MS)-based proteomics is vulnerable to peptide loss during final sample preparation. We evaluated whether ChocoTip desalting and decyl maltose neopentyl glycol (DMNG)-assisted reconstitution improve peptide recovery from HeLa digests corresponding to 10 ng of protein (∼50 cells). ChocoTip re...

Akane Omori, Eisuke Kanao, Yasushi Ishihama · Electrophoresis · 0 citations

Open access Jul 2026

A Multi-Omics Framework Reveals Phosphorylation-Dependent Control of Protein Interactions

A nested linear regression model is applied to pan-cancer data from 1,006 tumours and reveals a widespread coupling between phosphorylation and interaction-dependent protein abundance and provides a prioritized, structure-informed resource for characterizing the human phosphoproteome.

Kosuke Ogata, Emmanuel Matabaro, E. Aarts et al. · bioRxiv · 0 citations