Engineering the Metabolic Pathway of Escherichia coli for Efficient Biosynthesis of Lacto- N -tetraose
Lacto-N-tetraose (LNT), a core component of human milk oligosaccharides (HMOs), plays essential biological roles; however, its production remains challenging due to low conversion efficiency and the accumulation of intermediates. In this study, a systematic metabolic engineering strategy was applied to Escherichia co...