Perilla seed oil reshapes the rumen microbiome and increases fermentation end-products in vitro.
AIMS The effect of Perilla frutescens seed oil (PSO) on an in vitro rumen microbial ecosystem was evaluated by integrating fermentation measurements, microbiome profiling, metagenomics, and untargeted metabolomics. METHODS AND RESULTS Rumen inoculum was incubated for 24 h with a control TMR substrate (CK), TMR supple...