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Yi-Min Cai

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Sep 2026

Ancestral Sequence Reconstruction Synergized with Deep Learning Significantly Enhances the Thermophilicity and Thermostability of the Highly Active Alkaline Protease AprE

Molecular dynamics simulations revealed that AP291 exhibits reduced local structural fluctuations and enhanced conformational stability, clarifying the underlying stability-enhancing mechanism, and AP291 is promising for industrial biocatalysis.

Yi-Min Cai, Ya-Rui Bian, Yu-Ying Chen et al. · 0 citations
Jul 2026

Structure-informed engineering of a laccase with enhanced catalytic activity and thermostability for facilitating lignocellulosic biomass saccharification.

Lignocellulose is an abundant renewable feedstock for biofuels and value-added bioproducts, yet its efficient bioconversion is hindered by the recalcitrant lignin barrier. While laccases show great potential for lignin modification and delignification, their limited thermostability restricts their application in high-t...

Lei Zhao, Dianqing Liu, Yi-Min Cai et al. · 0 citations

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