Enhanced thermostability and structural integrity of dextransucrase via oxidized dextran-mediated modification.
Dextransucrase DsrD5 synthesizes low-molecular-weight dextran (~5 kDa) but has limited industrial utility because of poor thermal and pH stability. Here, DsrD5 was modified with oxidized dextran (ODX) to improve enzyme robustness while retaining the principal low-molecular-weight product profile. Ammonium sulfate enric...