Nanoporous gold electrode-assisted CRISPR/Cas12a electrochemical detection of synthetic methylated DNA models for breast cancer liquid-biopsy development
Circulating tumor DNA (ctDNA) methylation is a promising liquid-biopsy signal for early breast cancer, but low target abundance, fragmented cell-free DNA (cfDNA), and matrix fouling remain major barriers to electrochemical implementation. Here, a nanoporous gold (NPG) electrode-assisted CRISPR/Cas12a electrochemical as...