Metabolic engineering of Lacticaseibacillus paracasei alleviates post-acidification and reshapes metabolite profiles in probiotic yogurt
Post-acidification during storage limits the use of multiple probiotics in fermented dairy products. In this study, the ldh gene was deleted in Lacticaseibacillus paracasei Zhang (LCZ), resulting in 45% reduction in lactic acid production. Co-fermentation of the engineered strain with commercial starters effectively co...