Open access
Aug 2026
De Novo Design of a Protein Binder to Probe Gas Channel and Enhance the Oxygen Tolerance of [NiFe]-Hydrogenase.
[NiFe]-Hydrogenases display remarkable catalytic efficiency for H2 production and oxidation but are sensitive to O2, which severely restricts their biotechnological applications. The identities and functional relevance of gas channels leading to the buried [NiFe] active site remain elusive, hindering rational enzyme en...
Xuan Sun, Qi Xiao, Nan Zhang et al.
· Angewandte Chemie · 0 citations