Sep 2026
Mismatch-Engineered CRISPR Transistors for Ultra-Low-Frequency Single-Nucleotide Variant Detection
Accurate detection of low-frequency single-nucleotide variants (SNVs) in a high wild-type (WT) background remains challenging. Here, we report a mismatch-engineered CRISPR transistor (MECT) for rapid, amplification-free detection of mutant RNA. MECT integrates a Cas13a–crRNA recognition interface with a graphene fiel...
Chang Chen, Hongwenjie Ma, Han-Ting Xia et al.
· Nano letters (Print) · 0 citations