Author

Zhanzhi Liu

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Open access Aug 2026

Continuous Evolution System Based on Toxin–Antitoxin System Combined with Base Deaminase Accelerates the In Vivo Modification of Target Proteins

ABSTRACT To overcome the labor‐intensive nature of traditional directed evolution, the promoted Escherichia coli‐assisted continuous evolution (PEACE) system has been developed. PEACE 1.0 employs a cytidine deaminase‑T7 RNA polymerase (T7 RNAP) fusion for targeted mutagenesis and integrates a toxin–antitoxin selection...

Xinyu Zhang, Zhan-Zhi Liu, Ying Xu et al. · Advancement of science · 0 citations

Jul 2026

Rational engineering of cutinase for enhanced biodegradation of polyvinyl acetate and polyacrylates to control stickies in pulp and paper processing.

In the pulp and paper industry, residual sticky substances accumulate during white water circulation, forming deposits that cause paper defects and increase costs. Cutinase is a promising biocatalyst for mitigating stickies, but its industrial application is limited by inherent catalytic constraints. To address this, a...

Shiheng Chen, Zhisheng Wei, Sheng Wang et al. · Bioresource Technology · 0 citations