Structure-Optimized Asymmetric siRNA Increases Predicted AGO2 Binding Affinity to Augment Targeted MMP7 Gene Silencing Efficacy.
RNAi holds significant therapeutic potential, yet the clinical efficacy of siRNA drugs has been constrained by challenges in stability, targeted accumulation, and efficient loading into the RISC. To address this, we hypothesized that rationally extending or shortening the terminal base of the siRNA antisense strand cou...