Substrate-Oriented Channel Engineering of D-Amino Acid Oxidase for Efficient L/D-Phosphinothricin Resolution: Synergies of Geometric and Electrostatic Modifications.
D-Amino acid oxidase (DAAO) catalyzes the oxidation of D-phosphinothricin (D-PPT) to produce optically pure L-PPT, but low catalytic efficiency limits its industrial application. Here, a synergistic strategy combining geometric remodeling and charge engineering was applied to enhance the oxidation activity of our previ...