Zhifa Shen

Decoding Biomolecular Phase Separation: From Physiological Roles to Pathological Mechanisms and Therapeutic Biomaterials Design

ABSTRACT Biomolecular phase separation (BPS), encompassing liquid‐liquid, liquid‐gel, and liquid‐solid transitions, has emerged as a fundamental principle governing cellular organization and function. Dysregulation of these phase states acts as a pathogenic driver of diverse intractable diseases, including neurodegener...

Nonenzymatic detection and imaging of m6A-methylated RNA via proximity hybridization-initiated hybridization chain reaction with site-specific recognition.

Turning DNAzyme into a Stitch-to-Activate Biosensor for Single-Base-Resolution Molecular Diagnostics

The catalytic core of DNAzymes is highly sensitive to local structural perturbations. Conventional DNAzyme-based SNV sensing mainly relies on differences in hybridization stability between matched and mismatched targets. Here, we position the SNV immediately adjacent to the catalytic-core reconstitution junction of a...

Dual-tetrahedral DNA-enabled strand displacement amplification biosensor for cell imaging and clinical detection of miR-34a-5p in NAFLD.

Membrane-anchored DNA nanodevice with allosteric aptamer arms enables parallel probing and on-site drug delivery

A DNA nanodevice that anchors to cell membranes and releases chemotherapy only when three specific cancer markers are detected simultaneously is developed, establishing a versatile platform for logic‑controlled, multimarker‑guided cancer theranostics.