Integrative single-cell and spatial transcriptomic analysis reveals a lactate-driven crosstalk between NFATc4⁺ tumor cells and SPP1⁺ macrophages in glioblastoma.
BACKGROUND Glioblastoma (GBM) remains a lethal brain tumor with limited therapeutic options. Metabolic reprogramming, particularly lactate metabolism, plays a critical role in tumor progression and immune evasion. METHODS Here, we integrated single-cell RNA sequencing (scRNA-seq), spatial transcriptomics, and machine...