Ziran Zhai

Direct measurement and reconstruction of intact polyclonal IgG repertoires to preserve molecular and functional connectivity

Antibody function depends on the molecular pairing of antigen-binding Fab regions with Fc domains, linking antigen specificity to effector potential. However, antibody repertoire methods analyze these subunits separately, creating an inference problem in which Fc-Fab connectivity is lost. Here, we introduce a multi-lev...