Aug 2026· Diagnostics· Vol 16, pp. 2613· 0 citations· 46 references
Medicine
TL;DR
Evidence on next-generation sequencing (NGS) in melioidosis holds promise for improving melioidosis management, especially in resource-limited settings, despite cost and standardization challenges.
Abstract
Melioidosis, caused by Burkholderia pseudomallei, is a severe infectious disease with high mortality. Diagnostic delays due to conventional culture and serology limitations impact patient outcomes. This narrative review synthesizes evidence on next-generation sequencing (NGS) in melioidosis. NGS technologies encompass two main applications: metagenomic NGS (mNGS), which enables culture-independent detection directly from clinical samples, and whole-genome sequencing (WGS), which provides outbreak tracing and source attribution from cultured isolates. Resistance profiling detects antimicrobial resistance (AMR) determinants (e.g., penA mutations) to guide therapy. Recent 2025–2026 studies highlight new applications, including direct pathogen genome recovery from environmental samples. Despite cost and standardization challenges, integrating NGS into clinical workflows holds promise for improving melioidosis management, especially in resource-limited settings.
Abstract Carbapenem-resistant Acinetobacter baumannii (CRAB) is a major global public health threat. The World Health Organization (WHO) has classified CRAB as a critical priority pathogen. This threat is mainly attributed to its multidrug resistance, efficient nosocomial transmission, and limited therapeutic options....
Qiu-Ping Ye, Ya-Qun Liu, Kang Zhang et al.· Infection and Drug Resistanc...· 0 citations
Background Metagenomic next-generation sequencing (mNGS) increasingly detects Nocardia, but interpreting positive results remains challenging. We aimed to identify host factors and read thresholds that distinguish nocardiosis (ND) from colonization/contamination, and to characterize clinical features linked to immune s...
Ping Li, Xiao Yang, Yu-Xia Chen et al.· Frontiers in Cellular and In...· 0 citations
Introduction: Respiratory tract infections remain a leading cause of morbidity and mortality worldwide, burdening healthcare systems and promoting antimicrobial resistance. Accurate and timely diagnosis is vital for effective management and infection control. Over time, diagnostic modalities have evolved from microscop...
Saurabh Chhotalal Norris, Pushpa Ramjibhai Kateshiya, Binda Prakashbhai Pipaliya et al.· IP Indian Journal of Immunol...· 0 citations
Background Nontuberculous mycobacteria (NTM) are an increasingly common group of pathogens that remain challenging to diagnose and treat effectively. The lack of standardization of NTM management, from identification to antibiotic resistance prediction, results in imperfect correlations between treatment and outcomes....
A. Menon, C. Mariner-Llicer, A. M. Xet-Mull et al.· medRxiv· 0 citations
Delays in pathogen and antimicrobial resistance (AMR) profiling adversely affect patient outcomes. Next-generation sequencing (NGS) may complement conventional microbiology (CM), yet real-world value of NGS in pneumonia diagnosis remains unclear. We quantified concordance between a commercial NGS panel and CM for pat...
N. Sandybayev, R. Sarsenov, V. Strochkov et al.· Royal Society Open Science· 0 citations
Introduction: Meningoencephalitis (ME) is an inflammatory condition of the meninges and brain parenchyma, remains a major diagnostic challenge. Metagenomic Next-Generation Sequencing (mNGS) has emerged as a promising tool for unbiased detection of viral, bacterial, fungal, and parasitic pathogens often missed by conven...
Payal Khulkhule, Anil Agrawal, Amit R. Nayak et al.· Journal of Clinical and Diag...· 0 citations
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