Multifunctional MoO3-X Nanozyme-Hydrogel for Synergistic Photothermal Antibacterial Therapy and Enhanced Healing of Wounds Infected with Drug‑Resistant Bacteria.
Aug 2026· ACS Applied Materials and Interfaces· 0 citations· 49 references
Medicine
TL;DR
This work demonstrates a safe and effective strategy for combating MDR infections through the combined action of photothermal therapy and nanozyme catalysis, offering promising potential for clinical wound management.
Abstract
Multidrug-resistant (MDR) bacterial infections and recalcitrant biofilms remain intractable barriers to wound healing, with traditional antibiotics offering limited efficacy due to resistance and poor biofilm penetration. To address this unmet clinical need, we innovatively engineered a multifunctional composite hydrogel (MoO3-X@CG-ODP) by integrating oxygen-vacancy-rich MoO3-X nanozymes into a dynamic cross-linking network formed by L-glutamine-grafted chitosan (CG) and 3-aminophenylboronic acid-mediated oxidized dextran (ODP). The composite exhibits a stacked nanosheet morphology (∼240 nm), a high photothermal conversion efficiency of 49.70%, and peroxidase-like activity. Under 808 nm near-infrared irradiation, the hydrogel achieves >99% inhibition against MDR Escherichia coli and Staphylococcus aureus, along with effective biofilm eradication. In a murine wound model, the material significantly accelerated wound closure, up-regulated vascular endothelial growth factor (VEGF), down-regulated tumor necrosis factor-α (TNF-α), and maintained excellent biosafety. This work demonstrates a safe and effective strategy for combating MDR infections through the combined action of photothermal therapy and nanozyme catalysis, offering promising potential for clinical wound management.
A pioneering near-infrared activated antibacterial EPLGA/OHADA@HMCuS hydrogel was formulated by doping hollow mesoporous copper sulfide (HMCuS) into the three-dimensional spatial network structure formed between gallic acid-modified ε-polylysine (EPL-GA) and dopamine-engineered and oxidized hyaluronic acid (OHADA) and showcased favorable biocompatibility, sustaining cellular viability.
N. Wang, Wei Jiang, Feiyu Lu et al.· Biomaterials Advances· 0 citations
This work developed a synergistic single-platform strategy for precise regulation of diabetic wound microenvironments, providing a promising therapeutic alternative for refractory diabetic wound treatment.
Infected burn wounds are characterized by bacterial invasion, oxidative stress, and persistent inflammation, which severely impair tissue regeneration. Herein, we report a crosslinker-free, hydrogen-bonded cationic guar gum hydrogel (CBBM) co-loaded with berberine (BBR) and MnO2-coated black phosphorus nanosheets (BPNS@MnO2) for the microenvironment-adaptive treatment of infected burn wounds. The dynamic guar gum network endowed the hydrogel with injectability, self-healing ability, and conformal adaptability. BPNS@MnO2 exhibited pH-dependent enzyme-like activities, including OXD-like antibacterial activity under acidic conditions and SOD-/CAT-like ROS-scavenging activity under near-neutral conditions and endowed the hydrogel with NIR-triggered photothermal responsiveness. Moreover, NIR irradiation further enhanced the antibacterial efficacy and moderately enhanced BBR release from the hydrogel. In vitro, CBBM combined with NIR irradiation achieved potent antibacterial efficacy against S. aureus and E. coli under the tested conditions and reduced intracellular ROS levels. In vivo, the CBBM + NIR treatment accelerated infected burn wound healing, achieving a wound closure rate of 97.53 ± 2.01% by day 14, accompanied by reduced inflammation, enhanced collagen deposition, and increased expression of the angiogenesis-related markers VEGF and CD31. These results demonstrate that the CBBM hydrogel is a promising multifunctional guar gum-based dressing for infected burn wound healing by integrating local drug delivery, catalytic regulation, and photothermal activation.
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The antibacterial experiments results show that the combination of antibacterial and anti-inflammation mediated by TAPP/Mn3O4@CS-GA hydrogel can accelerate infected wound healing.
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The in vivo experiments and transcriptome analysis demonstrated that the combination of CTA hydrogel and NIR treatment significantly accelerated the healing process of MRSA-infected wounds by eliminating antibiotic-resistant bacterial infections, regulating inflammatory responses, promoting collagen deposition and angiogenesis.
Chenghao Li, Pengyuan Liu, Pei Cheng et al.· International Journal of Bio...· 0 citations
A visible-light-responsive organic nanoplatform (iTPyPXs/SCM) that improves ROS utilization for antimicrobial therapy while limiting excessive intracellular ROS and provides a safer and more effective biomaterial strategy for photodynamic therapy in infected wound healing is developed.
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