An lncRNA-aware single-cell analysis framework — retaining all detectable GENCODE v45 lncRNAs during highly variable gene selection — combined with donor-level validation that guards against pseudoreplication is presented, and the pseudotime screen is reported as a negative result.
BACKGROUND AND AIMS
Long non-coding RNAs (lncRNAs) are important regulators of cellular processes, but their analysis at single-cell resolution remains challenging because lncRNA prediction, quantification, cell-type-specific characterization and downstream functional interpretation are often performed using separate t...
Shuwei Yin, Yi Lu, Wenyu Yan et al.· Annals of Botany· 0 citations
Nominating DIO3OS and C9orf139 as candidate survival-associated lncRNAs in GBM is shown, to show how endpoint definition, selection optimism, and external validation determine which lncRNA associations survive scrutiny.
Minseon Kim, Seung-Kyoon Kim, Jaeil Han· Non-Coding RNA· 0 citations
A single-cell multiomics atlas of the human pancreas is presented, profiling over four million cells and nuclei from 57 donors across fetal development, adult homeostasis, and type 2 diabetes.
E. Mereu, D. Balboa, J. Liebig et al.· Cell Metabolism· 1 citation
Background Community-acquired pneumonia (CAP) is associated with marked immune dysregulation, but the peripheral T-cell transcriptional alterations linked to CAP remain incompletely defined. We aimed to characterize CAP-associated T-cell heterogeneity and identify candidate blood biomarkers by integrating single-cell a...
Hua-Chi Jiang, Xiao-Qi Fan, Shu-Fang Zhou et al.· International Journal of Med...· 0 citations
To profile unknown ncRNAs—“dark matter” in single cells, we developed dropTotal, a high-throughput droplet-based total RNA-seq method that uses dU-modified GAT primer with temperature-ramp hybridization and droplet merge barcoding to co-detect coding and non-coding transcripts with record sensitivity (>13,500 genes/cel...
Xu-Dong Liu, Wen-Jian Cao, Ya-Ting Pan et al.· bioRxiv· 0 citations
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