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Identification of Phloem Cell Transcriptome via Single-Cell RNA-Sequencing.

2026 · Methods in molecular biology · Vol 3066, pp. 133-142 · 0 citations
Medicine

TL;DR

An optimized protocol for isolating vasculature-enriched protoplasts from mature Arabidopsis thaliana leaves is presented and the basic pipeline for downstream analysis is introduced, providing a reliable and reproducible method for isolate high-purity phloem-enriched protoplasts.

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