Bridging the Bench and Bedside: Stem Cell Cultivation Approaches for Clinical Treatment

Abstract

Regenerative medicine largely depends on the clinical use of stem cells which are promise as a new therapeutic paradigm. For any stem cell therapy, large-scale cultivation of the stem cells will be necessary. Nevertheless, the translation of the findings from the bench to the bed will represent a challenging feat. In this review, the author emphasizes the issues related to the current status and difficulties regarding the clinical application of stem cell research. Since the focus of this paper is cultivation of stem cells for clinical purposes, this review has covered hESCs, iPSCs, MSCs, and HSCs. In addition, through this review, the author succeeds in presenting information about the current state of growing and engineering stem cells. This review is concerned with the process of designing cell therapy using modern engineering techniques that have made the translation from stem cell research bench to stem cell clinical bedside an interesting development field. In this study, we address the effects that engineering of the niche by 3D bioreactor, xenogeneic-free media, and genetic manipulation of cells have on clinical outcomes in the context of stem cell therapies for neurological, cardiovascular, orthopedic, and immunological purposes.t on the impact of genomic instability on stem cell quality and the regulatory implications.