One-Pot Isothermal Linear Ampliﬁcation and Cas12a-Based Nucleic Acid Detection

Selma Sinan Remy M. Kooistra Karunya Rajaraman Zeba Islam Damian Madan E. A. Nalefski Ilya J. Finkelstein

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TL;DR

CATNAP (Cas trans -nuclease detection of ampliﬁed products), a method that integrates isothermal linear DNA ampliﬁcation with Cas12a detection in a single reaction, bridges the sensitivity gap in CRISPR diagnostics while maintaining simplicity, making accurate disease detection more accessible in resource-limited settings.

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