2026· Methods in molecular biology· Vol 3017, pp.
169-184
· 0 citations
Medicine
TL;DR
This chapter outlines a comprehensive protocol for identifying and functionally characterizing wheat lncRNAs under stem rust infection, and presents a comprehensive pipeline for identification and functional interpretation of novel regulatory lncRNAs, making it applicable for exploring ncRNA-mediated regulations in rust-infected wheat and related pathosystems.
ABSTRACT Long non‐coding RNAs (lncRNAs) play essential roles in plant growth and development. However, their expression variability and contribution to phenotypic changes during the domestication of fruit crops remain unclear. Here, we generated approximately 3.5T of strand‐specific RNA sequencing data from wild, landr...
Bo-Bo Song, Peilin Han, Jia-Ming Li et al.· Advancement of science· 0 citations
MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression through a sequence-specific recognition of their targets, leading to degradation or inhibition of translation. In the case of plant miRNAs, they have been involved in a multitude of biological processes, from developmental processes to environmen...
Josefat Gregorio-Jorge, Carlos Alberto Minor-Merino, Carmina Xicohténcatl-Ordoñez et al.· Horticulturae· 0 citations
Current knowledge on AS and lncRNAs is summarized and their integrated roles in improving abiotic stress tolerance in plants are highlighted, enabling regulatory flexibility and stress resilience.
A comprehensive population-level atlas of heat-responsive lncRNAs during wheat grain filling and highlights MSTRG.64082 as a promising candidate locus for breeding heat-tolerant wheat varieties is provided.
Wei-Shuang Zhao, Hui-Qiang Wang, Qiang Li et al.· BMC Plant Biology· 0 citations
This study systematically reanalyzed strand-specific RNA-seq data to characterize circRNAs responsive to simultaneous heat and drought stress, suggests their potential as ceRNAs based on predictive analysis, and lays a foundation for future experimental validation of circRNA-mediated regulation.
B. Hajieghrari, M. T. Giglou· Zeitschrift für Induktive Ab...· 0 citations
The main miRNA data repositories were identified and characterized, as well as the main computational methods for predicting miRNAs in plants, categorized as comparative, non-comparative, and based on next-generation sequencing data.
João Victor Da Paixão Reis Soares Dias, Cláudia Araújo Bastos· Journal of Interdisciplinary...· 0 citations
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