A Fluorescent Probe Based on Carbazole and Benzoindole for On‐Site Detection of Sulfite

Abstract

Sulfites are widely used as preservatives and antioxidants in food, but excessive intake poses health risks. Therefore, rapid, sensitive, and on‑site detection of sulfites is essential for food safety. In this work, a cationic small‑molecule fluorescent probe was synthesized via the condensation of N ‑ethylcarbazole (electron donor) and a benzoindolium ion (electron acceptor). It exhibits a D–π–A architecture with an intramolecular charge transfer (ICT) effect, showing red fluorescence at 617 nm. Upon reaction with HSO 3 − through Michael addition at the conjugated C═C bond, the ICT pathway is blocked, leading to fluorescence quenching. The probe displays excellent selectivity for HSO 3 − (limit of detection = 0.1 µM). Significantly, it can be simply deposited onto filter paper to fabricate easy‐to‐use test strips. Under a 365 nm UV lamp, the test strips exhibit a visible color change from red to blue with increasing HSO 3 − concentration, allowing naked‑eye detection. Coupled with a smartphone color‑reading application, a linear relationship between the blue‐to‐red intensity ratio (B/R) and HSO 3 − concentration enables rapid on‑site quantitative analysis. The test strips were successfully applied to detect residual HSO 3 − in various dried fruit samples. This work provides a simple, low‑cost, and practical method for detecting sulfite levels in food and environmental samples.