Energetic coupling of an active site residue in penicillin-binding protein 2 from Neisseria gonorrhoeae with a resistance-associated conformational switch in the β3-β4 loop

Open access Aug 2026

Mutation-induced heterogeneity of the β7-β8 loop of the Staphylococcus aureus class A sortase leading to enhanced catalytic efficiency characterized by NMR and enzyme kinetics

NMR data, in combination with enzymatic assays using active variants confirmed differences in the active, apo states of these enzymes, and provided additional atomic detail regarding the importance of the P94 residue in saSrtA substrate recognition.

Erich G. Walkenhauer, Noah Cox-Tigre, M. Chaubey et al. · bioRxiv · 0 citations

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Aug 2026

Deciphering the Role of the Nonactive Site Ancillary Residues in Maintaining the Activity and Substrate Specificity of the OXA-232 β-Lactamase.

Findings establish S118 and D159 as essential for core catalysis and structural integrity, with V120 and L158 modulating substrate-specific turnover and orientation.

Ajith Tejavath, Bayomi Biju, Aditya Prasad Panda et al. · ACS Infectious Diseases · 1 citation

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Aug 2026

Mechanistic Roles of Conserved Active-Site Residues in OXA-48 Carbapenem Hydrolysis.

Results support a model in which conserved hydrophobic active-site residues optimize the geometric and electrostatic environment required for efficient carbapenem deacylation and turnover, and provide mechanistic insight into OXA-48 catalysis that may be exploited to design inhibitors targeting the enzyme.

DorisMia Taylor, D. Ngango, Jia-Yi Fan et al. · ACS Infectious Diseases · 0 citations

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Review Open access Sep 2026

Escherichia coli with penicillin-binding protein 3 insertions: implications for newer β-lactam/β-lactamase inhibitor combinations

ABSTRACT Escherichia coli with penicillin-binding protein 3 (PBP3) insertions appeared in the early 2000s and expanded during the 2010s. The YRIN and YRIK variants predominate globally, while other insertion types remain rare. These insertions frequently co-occur with carbapenemases (NDM-5 and OXA-181) and high-risk cl...

Johann D. D. Pitout, G. Peirano, Y. Matsumura et al. · Antimicrobial Agents and Che... · 0 citations

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Sep 2026

Unveiling the structural details of the nucleotide-binding protein of an antimicrobial peptide transporter from non-typeable Haemophilus influenzae.

Non-typeable Haemophilus influenzae (NTHi) is an opportunistic extracellular pathogen associated with various respiratory diseases. To combat host antimicrobial peptides, NTHi has developed a specialized transporter named Sap (sensitivity to antimicrobial peptide). Within this transporter, the HiSapF component function...

Kalyan Ghosh, S. P. Kanaujia · International Journal of Bio... · 0 citations

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Aug 2026

A Single Substitution of Valine to Glutamic Acid in the Omega-Like Loop Introduces a Penicillinase-Like Activity in MSMEG_6194 by Compromising its Role as DD-Carboxypeptidase

Findings suggest that MSMEG_6194 act as a DD-carboxypeptidase and illustrate how a single amino acid change can alter catalytic preference, shedding light on the evolutionary transition from DD-carboxypeptidases to beta-lactamases in mycobacteria.

Aditya Prasad Panda, Debasmita Chatterjee, Anik Roy et al. · Current Microbiology · 0 citations

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