Analytical methodology for unveiling the physical effect of detergents on the function of full-length membrane proteins using a single-molecule tracking system

Effects of Detergent Selection on Stability of a Recombinant Integral Membrane Protein

Characterization and structural analysis of integral membrane proteins (IMPs) require detergents that can efficiently disrupt the lipid bilayer while maintaining the native conformation, solubility, and stability of the protein. Selecting an appropriate detergent therefore represents a critical bottleneck in membrane p...

A generalisable method for the purification and biophysical characterisation of bacterial membrane receptors

A generalizable workflow for producing soluble receptor domains from membrane proteins is established and validated HasR constructs suitable for downstream ligand-screening applications, including aptamer and nanobody discovery.

Analytical Ultracentrifugation for Studying the Oligomeric State and Protein-Protein Interactions of Membrane Transporters.

Expression and Purification of Integral Membrane Enzymes for Functional and Structural Studies.

Together, the methods described here demonstrate that careful selection via thorough screening, and optimization of expression and purification conditions, are an invaluable investment of time, ultimately yielding the best target not only for successful high-resolution structural analyses-X-ray crystallography and cryo...

Tools to investigate class B1 GPCR conformational landscapes with cryo-EM and beyond.

G protein-coupled receptors (GPCRs) are membrane proteins that act as signal transducers across cell membranes. Class B1 GPCRs, a subset of 15 receptors activated by peptide hormones, are involved in important physiological processes and diseases, making them a popular target for drug development. GPCRs are dynamic pro...

An Excelled Trifluorinated Probe for GPCR Conformational Quantification

G protein-coupled receptors (GPCRs) represent the largest membrane protein family in the human body and the major targets of FDA-approved medications. Unlike some other physiological proteins, GPCRs are highly plastic and often exist as collections of conformational ensembles in the environment to exert their function....