Gene ontology and KEGG enrichment analyses were performed to identify DEG-regulated biological functions and pathways and the PPI network showed six hub genes: CCND1, CDH1, ESR1, FN1, IL6 and PPARG as the top key genes.
Abstract
The molecular basis of TNBC development is poorly understood. Therefore, it is of interest to identify key genes and pathways involved in TNBC development and progression using a systems biology approach, followed by experimental validation. Gene ontology and KEGG enrichment analyses were performed to identify DEG-regulated biological functions and pathways; a PPI network was constructed using the STRING online database. The expression and prognostic value of the hub genes were validated by TCGA survival analysis, resulting in the identification of 727 DEGs (473 downregulated and 254 upregulated) in TNBC samples. The GO and KEGG analyses indicated that the DEGs were mainly related to cell adhesion and tumorigenesis and the PPI network showed six hub genes: CCND1, CDH1, ESR1, FN1, IL6 and PPARG as the top key genes. These were validated by real-time PCR in the TNBC cell line using a non-TNBC cell line as a calibrator and the obtained results were in accordance with the bioinformatics data
This study provides a hypothesis-generating framework for future biomarker-guided combination strategies, which requires validation in HER2-positive cohorts and experimental models.
Feng Wang, N. Mottaghi-Dastjerdi, Fatma Altameemi et al.· Iranian Journal of Basic Med...· 0 citations
Triple-negative breast cancer (TNBC) is a highly aggressive and treatment-resistant subtype, lacking HER2, progesterone, and estrogen receptors. Systemic chemotherapy remains the primary treatment due to the absence of molecular targets, often leading to poor prognosis and high recurrence. This study used an integrated...
Nazia Zarin, Tasnim Hosen Tanha, Mimuna Nishad et al.· Bioinformatics and Biology I...· 0 citations
BACKGROUND
Oral squamous cell carcinoma (OSCC) is among the most common head and neck cancers, representing about 90% of all cases. Despite advances in treatment, the prognosis remains poor, mainly owing to the complex immunosuppressive tumor microenvironment.
METHODS
The GEO2R tool was used to screen differentially...
Mohammad Hossin Shushizadeh, Niloufar Sadat Kalaki, Tahmineh Mohammadi Chamardani et al.· Cancer Treatment and Researc...· 0 citations
This study identified five lung cancer-associated hub genes and provided preliminary evidence that resveratrol may modulate their expression, and provides a multi-level molecular framework for further investigation of resveratrol-responsive networks in lung cancer.
Jing-Hua Yang, Han-Xiu Wei, Jie Li et al.· Cell Biochemistry and Biophy...· 0 citations
Cystic Fibrosis, an inherited disorder, is primarily found in white American society. It arises due to alteration in the CFTR gene. Several pieces of evidence insight oxidative stress as one of the primary reasons behind this disorder. Thus, with the aim of finding the physiopathology of this disorder and its associati...
Aurobinda Das, Suresh Kumar Panda, Debakanta Sahoo· Journal of Science & Tec...· 0 citations
PURPOSE
There is few research on which genes play an important role in tumors without lymph metastasis. This study aimed to identify candidate molecular alterations preferentially associated with N0-stage LUSC.
METHODS
we conducted a comprehensive bioinformatics analysis using publicly available The Cancer Genome Atl...
Marzyeh Alipour, M. Moghanibashi, S. Naeimi et al.· Computers in Biology and Med...· 0 citations
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