[Rational design and molecular engineering of a chitosanase for preparing specific chito-oligosaccharides with degree of polymerization≥5].

Engineering CBM of β-Mannanase from Paenibacillus to Alter Product Chain Length for Preparing Mannan Oligosaccharide Prebiotics.

Biomass polysaccharides are explored as inexpensive raw materials for preparing value-added products, such as mannan oligosaccharides (MOS). However, the varied composition of MOS and the underlying mechanism have not been studied thoroughly yet. Here a β-mannanase from Paenibacillus sp. was engineered for hydrolyzing...

Enhanced enzymatic hydrolysis of chitosan via MOF-mediated activation of chitosanase.

Chitosanases are attractive biocatalysts for producing bioactive chito-oligosaccharides (COS), but their applications are limited by insufficient catalytic efficiency, poor stability, and challenging recovery. Although many chitosanases can be activated by metal ions, the direct addition of free ions into reaction syst...

Influence of a Thermostable Fungal Xylanase Combined With Multi-Enzyme Complexes on the Structure, Physicochemical, and Functional Properties of Dietary Fiber From Hawthorn Pomace.

Effects of xylanase (XynA) in combination with various fibrolytic enzymes on the extraction of soluble dietary fiber (SDF) from hawthorn pomace and on the modification of dietary fiber (DF) were investigated. A high-yield fungal strain (Thermomyces lanugiosus Q231) producing a thermostable xylanase (XynA) was screened,...

Rheology-Guided One-Pot Production of Mixed Xylo-/Manno-Oligosaccharides (XMOS) from Dual-Polysaccharide Biomass via Glutamic Acid Catalysis

A mixture of xylo-/manno-oligosaccharides (XMOS) was produced from Gleditsia sinensis peel (GSP) and seed endosperm (GPP) using glutamic acid (GA) catalysis. An enzymatic hydrolysis-HPLC method was established to quantify xylo-oligosaccharides (XOS) and manno-oligosaccharides (MOS) in XMOS. Rheological analysis revea...

Enzyme-assisted extraction, structural characterization, and bioactivities of oligosaccharides from jackfruit shreds

Mechanochemical Functionalization of Polysaccharides via Catalytic Esterification for the Preparation of Superabsorbent Materials for Plant Growth Applications

In this study we have developed a new approach to synthesize superabsorbent materials via derivatization of polysaccharides of soybean waste (okara) and banana fibres using mechanochemistry. These polysaccharides, in equal weight ratio, were derivatized with succinic anhydride (SA) in the thermo-mechanical reactive ext...