Synthesis of some Novel Pyrazole-derivatives and Evaluation of their Biological Activity as Putative Anti-inflammatory and Antioxidant agent

Abstract

Compounds containing pyrazole have shown promise as antioxidants and anti-inflammatory agents because they may be able to block p38α MAP kinase, a crucial therapeutic target in inflammation. The current study sought to synthesize a variety of pyrazole compounds using different synthetic procedures, characterize the synthesized diverse library of pyrazole compounds using spectroscopic techniques such as IR, NMR, and mass spectroscopy, and assess a number of novel pyrazole compounds for their anti-inflammatory activity in vitro using the albumin denaturation inhibition method and their anti-oxidant effect using the DPPH colorimetric technique at a concentration of 1mM to gauge their capacity to scavenge free radicals. The anti-inflammatory properties of the active compounds were confirmed by cell-based assays that showed dose-dependent inhibition of inflammatory markers. When compared to current anti-inflammatory drugs, the most promising compounds showed good cytotoxicity and selectivity profiles. These results demonstrate the potential of pyrazole-based anti-inflammatory and antioxidant moieties as innovative anti-inflammatory drugs. To assess their effectiveness and safety in pertinent disease models, more lead optimization and in vivo research are necessary. This work highlights the significance of pyrazole scaffolds in medicinal chemistry and advances the field of anti-inflammatory drug discovery.