TL;DR
A detailed protocol to analyse β-glucan oligosaccharides released from fungal and plant cell walls using thin-layer chromatography (TLC) and linkage-specific glucanases is provided and is a cost-effective and less time-consuming approach to analyze β-glucan oligosaccharides.
β-1,6-Glucan is a functionally crucial polysaccharide of the fungal cell wall, although typically less abundant than β-1,3-glucan and chitin, its content, chain length, and branching vary considerably among species. Structurally, it serves as a covalent cross-linker tethering the external mannoprotein layer to the inte...
Cell wall-degrading Carbohydrate-Active enZymes (CAZymes) have substantial potential for biological control of crop diseases by targeting the cell walls of filamentous plant pathogens (fungi and oomycetes) and could substitute for environmentally hazardous pesticides. The cell walls of fungi and oomycetes share common...
The 1988 papers by Mauch, Mauch-Mani, Hadwiger and Boller on pea antifungal hydrolases captured a simple but powerful idea: plants can attack pathogens by directly targeting the structural polymers that hold their cell walls together. At that time, chitinases and β-1,3-glucanases were known to accumulate after infectio...
Plant cell wall fragments released during pathogen attack can act as signalling molecules that trigger immune responses. Successful pathogens have potentially evolved diverse strategies to evade host recognition, including minimizing the accumulation of cell wall-derived elicitors. However, the mechanisms underlying th...
We carried out carbohydrate composition and carbohydrate linkage analyses on Neurospora crassa cell walls from vegetative hyphae, conidia (asexual spores), perithecia (female mating structures), and ascospores (sexual spores). We show that the composition and structure of the cell wall undergo dramatic changes during t...
The root extracellular trap (RET) is a network formed by root-associated, cap-derived cells (AC-DCs) and their mucilaginous secretions. The RET is located at the root tip where it establishes a protective interface against abiotic and biotic stresses. The RET mucilage is mainly composed of complex cell wall polysacchar...
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