Evaluation of MTHFR Gene Polymorphisms (C677T and A1298C) as Molecular Predictors of Recurrent Pregnancy Loss
TL;DR
It is suggested that certain polymorphisms in the MTHFR gene may contribute to the risk of recurrent miscarriage, although their impact appears to be limited in the studied population.
Abstract
Miscarriage is one of the most significant problems faced by women during pregnancy, which in turn leads to numerous psychological and physical complications affecting the mother’s health; however, the main cause of this condition remains unclear. The aim of this study was to investigate the relationship between polymorphisms in the methylenetetrahydrofolate reductase (MTHFR) gene and recurrent miscarriage in women in Anbar Governorate. A case-control study was conducted involving 70 women with recurrent miscarriage and 20 healthy women as a control group. Genomic DNA was extracted from peripheral blood samples, and the targeted genetic polymorphisms were analyzed using polymerase chain reaction (PCR) techniques. The frequencies of genotypes and alleles were compared between the two groups, and statistical analyses were performed to assess potential associations. The results showed differences in the distribution of certain genotypes and alleles between the patients and the control group; however, these differences did not reach statistical significance. These findings suggest that certain polymorphisms in the MTHFR gene may contribute to the risk of recurrent miscarriage, although their impact appears to be limited in the studied population. The study recommends further research using larger samples and from different ethnic groups to clarify the role of genetic variants in the MTHFR gene in causing recurrent miscarriage and to develop clinical methods for the early prediction of pregnancy loss.