Deciphering Gamete Differentiation in Ostrea edulis using Laser Capture Microdissection and RNA-seq

Spatiotemporal Profiling of the Marchantia Sporophyte Reveals Ancestral Meristem Module and Dynamic Epigenetic Reprogramming during Early Embryogenesis

This work generated a spatiotemporally resolved transcriptomic atlas of Marchantia sporophyte development using laser-assisted microdissection coupled with RNA sequencing and uncovered distinct gene expression programs associated with early embryogenesis, sporogenous specification, and late tissue differentiation.

Time-resolved transcriptomics reveals sex-specific programs of gametogenesis and post-spawning gonadal remodeling in the sea cucumber Apostichopus japonicus.

The sea cucumber Apostichopus japonicus is an important mariculture species in East Asia, yet the temporal programs that distinguish female and male gonadal development remain incompletely resolved. Histological staging and RNA sequencing were combined to profile female and male gonads across five histologically define...

Comparative genomics and adult-female transcriptomics prioritize physiological candidate pathways in the aquatic acentropine moth Paracymoriza distinctalis.

Freshwater-associated life histories are rare in Lepidoptera, and the physiological systems accompanying them remain poorly resolved. We integrated adult-female whole-body RNA-seq from Paracymoriza distinctalis (n = 3) and three terrestrial crambids (n = 3, 3, and 2) with focused comparative genomics to prioritize test...

High temperature disrupts sporocyte development in Camellia meiocarpa.

Integrative analysis of Smart-seq2 and DIA proteomics reveals dynamic formation of the Balbiani body during oocyte development in leopard coral grouper (Plectropomus leopardus)

Membraneless RNA granules play important roles in germ cell development, yet their molecular composition and developmental dynamics remain poorly understood in fish. In this study, we investigated the Balbiani body (BB) and a nuage-associated nucleus-containing region (NNR) in oocytes of leopard coral grouper usi...

Temporal, genome-scale analysis of Myxococcus xanthus developmental fate in a mixed population

Myxococcus xanthus bacteria form aggregates when starved on solid surfaces and some cells differentiate into spores. Studies of mutants in monoculture have advanced knowledge of this multi-cellular developmental process, but our understanding of the genetic determinants is incomplete. To assess gene function genomewide...