TL;DR
This chapter describes synthetic gene circuit-based methods for applying synthetic condensates in E. coli to regulate transcription and translation and demonstrates how engineering synthetic condensates offers a new layer of cellular control for synthetic biology.
This review aims to establish a foundation for rigorous, reproducible, and conceptually coherent research in condensate biology of plants and beyond, with emerging implications for crop genetic improvement and synthetic biology applications.
Liquid-liquid phase separation (LLPS), an interesting process in which a homogeneous solution demixes into solute-rich and solute-poor phases, serves as a ubiquitous mechanism for the formation of biomolecular condensates in living cells. Biomolecular condensates, also known as membraneless organelles, play critical ro...
Biomolecular self‐assembly is ubiquitous in nature, encompassing both ordered and disordered structures to create sophisticated superstructures essential for complex biological functions. Protein and peptide condensates formed via liquid–liquid phase separation (LLPS) are characterize by disordered assembly, gaining si...
It is found that affinity governs the phase boundary, resistance to chemical perturbation, and molecular mobility of condensates in vitro and in human cells and is shown to be a quantitative determinant of condensate phase behavior, internal dynamics and biochemical output.
The formation of active and catalytic biomolecular condensates is critical to orchestrate intracellular biochemical reactions and cellular functions. Synthetic analogues that mimic such behaviors are typically constructed via liquid-liquid phase separation that generates liquid coacervate-based droplets, which promote...
The results demonstrate that orthogonally assembled structures can serve as cellular substructures for active complex coacervate-based synthetic cells, broadening the existing arsenal of tools to bestow these rudimentary synthetic cells with more life-like properties.
We use cookies to run the site and, with your consent, for analytics and to show ads. See our Cookie Policy.
