A high-fidelity intestinal model derived from an in-house porcine induced pluripotent stem cell line is developed, effectively replicating the viral life cycle and its pathogenesis of the porcine epidemic diarrhea virus.
Abstract
Simple Summary Porcine epidemic diarrhea virus causes significant economic damage to the global swine industry. However, traditional research often relies on non-porcine cell lines or costly animal trials, which fail to accurately mimic natural infection processes. To address this limitation, we developed a high-fidelity intestinal model derived from an in-house porcine induced pluripotent stem cell line. Over the differentiation period, these cells formed a complex tissue containing intestinal stem cells, goblet cells, and mature enterocytes with a functional barrier. This model is permissive to this virus, effectively replicating the viral life cycle and its pathogenesis. Infection induced an immune response and structural fragmentation of the tight junctions. This platform offers a scalable, ethically sustainable tool for screening antivirals and gut supplements, bridging the gap between laboratory research and veterinary applications to improve animal health.
The aim of this set of experiments was to refine a ribonucleoprotein-based editing approach tailored specifically for pig intestinal organoids, with maximum editing up to 100 percent per IO.
Simple Summary Diarrhea triggered by enterotoxigenic E. coli F18 after weaning inflicts considerable economic damage on the pig industry. As extracellular vesicles, exosomes participate in cell-to-cell signaling and show promise as biomarkers for various autoimmune conditions. However, no prior work has examined the bi...
Yi-Feng Wang, Yi-Han Chen, Ming-an Sun et al.· Animals· 0 citations
Introduction Critically low blood donation rates and recurrent shortages in the blood supply have become pressing challenges in transfusion medicine. Xenotransfusion using porcine red blood cells (pRBCs) represents a promising alternative; however, risks of immune rejection and infection necessitate the development of...
Tra My Tran, Hyeon Ho Lee, T. H. Hwang et al.· Frontiers in Bioengineering...· 0 citations
Enteric bacterial infections, notably shigellosis and salmonellosis, represent a staggering global health burden. Vaccine development is severely hampered by host-restriction, creating a persistent "translational gap" where preclinical data fails to correlate with human outcomes. Traditionally, model selection has been...
Hemanta Koley, Arindam Mukherjee, A. Mukhopadhyay et al.· Vaccine· 0 citations
Simple Summary Studying porcine rotavirus and developing effective interventions can be challenging without a reliable laboratory model that mimics a pig’s gut. We developed a novel culture system by growing miniature, simplified versions of the pig small intestine, called organoids, from neonatal piglet tissue. We suc...
Huangsiwu Wei, Wen-Jie Wu, Mao-Kang Liu et al.· Animals· 0 citations
ABSTRACT While efforts to eradicate poliovirus are ongoing, non-polio enteroviruses (NPEV) continue to pose a substantial public health threat. NPEVs cause a wide range of diseases, from mild self-limiting infections to severe respiratory, neurological, and cardiac complications, with the highest burden occurring in ch...
A. K. Pereirinha da Silva, Bjoern Meyer, Lisa Bauer· Journal of Virology· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
