A rapid and sensitive HiFi-LAMP assay for the detection of varicella-zoster virus.

Abstract

Varicella-zoster virus (VZV) causes chickenpox and herpes zoster, posing a heavy global disease burden. Real-time quantitative polymerase chain reaction (qPCR) was widely used for VZV detection but requires sophisticated equipment and well‑equipped laboratories, limiting its use in primary care and resource‑limited settings. Here, we reported a probe‑based high‑fidelity loop‑mediated isothermal amplification (HiFi‑LAMP) assay targeting the VZV ORF62 gene. The assay showed high specificity without cross‑reactivity with nine common human pathogens, and high sensitivity with a limit of detection (LOD) of 65 copies per 25 μL reaction. The assay can be completed within 30 min. The assay showed complete concordance with qPCR for 53 tested clinical specimens. This rapid, sensitive and equipment-friendly assay shows potential for point-of-care detection of VZV.