Results indicate that snRNA-seq recapitulates the dynamic changes associated with injury as previously reported using single-cell methods and outperforms these approaches in the representation of cell types that are sensitive to processing, thereby highlighting its utility for high resolution analysis of heterogenous tissues such as lung.
This review systematically integrates current evidence from sc/snRNA-seq studies, with a primary focus on brain-intrinsic mechanisms after ischemic stroke, to provide a structured synthesis of how sc/snRNA-seq has advanced the understanding of ischemic stroke biology.
Chang-Qing Mu, Yu-Chuan Ding, Alexander Weiss et al.· Frontiers in Neurology· 0 citations
INTRODUCTION
Acute kidney injury (AKI) is a severe clinical syndrome associated with high morbidity and mortality, and it possesses high risk of progression to chronic kidney disease (CKD). Renal infiltration of inflammatory cells, particularly macrophages, plays a key role in driving the progression from AKI to CKD....
Liuyan Xiao, Yinyi Long, Xue Hong et al.· Journal of Advanced Research· 0 citations
Background Pseudomonas aeruginosa pneumonia triggers a complex, dynamic host response, yet the cellular coordination of inflammation and repair remains poorly defined. Methods We performed time-resolved single-cell RNA sequencing (scRNA-seq) on murine lungs across five timepoints (0–96 h post-infection) and integrated...
Fu-Liang Zong, Yan Wang, Ling-Fei Hu et al.· Frontiers in Immunology· 0 citations
Suspension mass cytometry can measure 50 or more markers per cell and is not impacted by cellular autofluorescence, making it an appealing platform for comprehensive phenotyping of single cells in tissue with high innate fluorescence, such as brain. However, this organ is challenging to reduce to a single cell suspensi...
Caroline E. Roe, Chiara Miller, Nidia Calero et al.· bioRxiv· 0 citations
Introduction Early brain injury (EBI) following subarachnoid hemorrhage (SAH) critically determines neurological outcomes, yet the cellular drivers remain unclear. Methods To systematically dissect acute pathogenic programs, we performed single-cell RNAsequencing of CD45+ immune cells isolated from a mouse model of SAH...
Kai-Kai Wang, Chang-Ming Liu, Hai-Feng Chu et al.· Frontiers in Neuroscience· 0 citations
The dissociation of tissue into single-cell suspensions for analysis aids in understanding what is happening at the cellular level in the tumor microenvironment. A longstanding challenge in obtaining and utilizing primary cells is producing high-quality cell suspensions that preserve cell characteristics for downstream...
Ryann Ray, Wei-Zen Wei, J. Jacob· Journal of Visualized Experi...· 0 citations
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