Innovative reproductive technologies for in vitro preservation and utilization of female gametes from domestic birds (review)

Abstract

The swift reduction in the biodiversity of farm birds and their wild relatives necessitates the development of effective in vitro preservation methods. Creation of cryobanks for long-term storage of avian reproductive cells represents a relevant strategic solution to this problem. However, to date, effective protocols for cryogenic preservation have been developed for bird spermatozoa only. Egg cryopreservation in birds remains impossible, which leads to loss of genetic information, namely nuclear and mitochondrial DNA of female birds. The utilization of the latest advances in production and cultivation of primordial germ cells (PGSs) and embryonic gonadal germ cells (GGCs) is a viable alternative for preserving the gene pool of birds. The advantage of protocols using GGCs is the early determination of the donor’s sex and targeted in vitro preservation of female reproductive material. Furthermore, there is a possibility of low-temperature intraovarian preservation of female bird gametes. The use of frozen-thawed ovarian tissues of both embryonic and postnatal origin as future transplants is being actively developed. The success of using donor reproductive material largely depends on the recipient preparation (sterilization). The world’s leading laboratories have achieved impressive results in producing offspring of both sexes using donor reproductive material. This review highlights the progress made in the developing technologies for the in vitro preservation and use of avian reproductive material: embryonic cells, donor ovarian tissues, and tissue transplantation to recipients. The development of biotechnological methods for manipulation with donor material of female birds will contribute to achieving the goal of the world scientific community, which is to prevent the loss of biodiversity of domestical birds, and will be further applied in the restoration of avian breeds and species that are currently at risk of extinction. An additional argument for the importance of developing these technologies is the significant economic advantage in reconstructing endangered breeds, compared to using only cryopreserved semen when conducting multiple backcrosses with a breed close in phenotype.