Keeping pace with the pulse of life

Open access Oct 2026

Interleaving SLIDE microscopy

Two-Photon microscopy (TPM) is an intravital imaging technique with deep tissue penetration, high three-dimensional resolution and low photobleaching. However, imaging speeds have traditionally been limited because images are generated by point scanning. Recently, Spectro-Temporal Laser Imaging by Diffractive Excitatio...

C. Stock, Gregory Berg, Matthea Thielking et al. · Frontiers in Photonics · 0 citations

Open access Sep 2026

Pulsed 978 nm swept-source laser for high-speed multiphoton microscopy.

Spectro-temporal laser imaging by diffractive excitation (SLIDE) enables two-photon microscopy (TPM) with 2D imaging rates in the kHz range and is capable of performing real-time volumetric imaging at video rates. However, the application of SLIDE microscopy is constrained by its available excitation wavelength. To dat...

S. Reinhards, T. Kutscher, K. Goodwin et al. · Optics Express · 0 citations

Open access Sep 2026

A custom two-in-one HIST and line-scanning confocal excitation module

Fluorescence microscopy applications often require specialized instruments that are optimized for different experimental goals. Here, we present a reconfigurable microscopy module that integrates highly inclined swept tile (HIST) illumination for high-sensitivity single-molecule imaging and line-scanning confocal micro...

Christian Arthur, D. Milkie, Andrew J. Ulmer et al. · bioRxiv · 0 citations

Review Open access Aug 2026

Beyond single cells: Ten years of commercial lattice light-sheet microscopy.

Lattice light-sheet microscopy (LLSM) enables gentle, rapid, volumetric fluorescence imaging of living biological systems. The technique employs Bessel beam interference to produce a light-sheet which retains its thickness over a relatively large field-of-view compared to other light-sheets. In the decade since its com...

Helena L. E. Coker, David S. Corcoran · Journal of Microscopy · 0 citations

Conference Open access 2026

miEye and microEye: An open-source ecosystem for benchtop single-molecule localization microscopy and single-particle tracking

Super-resolution fluorescence microscopy has emerged as a powerful tool for investigating biological structures at the nanoscale, well beyond the diffraction limit of classical optical imaging. Among super-resolution techniques, single-molecule localization microscopy achieves this by sequentially imaging and localizin...

M. N. Alsamsam, A. Kopu̅stas, M. Tutkus · EPJ Web of Conferences · 0 citations

Oct 2026

Single-Objective Light-Sheet Microscopy Allows Long-Term, High-Resolution In Vivo Brain Imaging of Drosophila

In vivo imaging of dynamic subcellular brain structures is key to understanding several phenomena in neuroscience. However, in the fruit fly, its implementation has been hindered by a trade-off among spatial resolution, speed, photobleaching, phototoxicity, and setup complexity required to access specific target regi...

Francisco J. Tassara, M. Barella, Tomás Sebastián Fernández Troncoso et al. · ACS Photonics · 0 citations