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Sep 2026
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Aflatoxin contamination, especially aflatoxin B1 (AFB1), is a major global health concern. There is an urgent need to develop rapid, highly sensitive and specific sensing platforms. In this study, two fluorescence aptasensors were developed for AFB1 detection. The first used quantum dots (QDs) combined with aptamers as...
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· In Analysis · 0 citations
Sep 2026
Universal CRISPR/Cas12a-G4 DNAzyme biosensing platform for rapid detection of nucleic acid and non-nucleic acid targets.
The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) system enables sensitive and specific biomolecular detection due to its programmability, high fidelity, and signal amplification. Herein, a novel universal CRISPR/Cas12a-G4 DNAzyme-TMB (Cas-GT) enzymatic biosensi...
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Sep 2026
An aptamer-regulated CRISPR/Cas12a system based on split crRNA for theophylline and tobramycin detection.
Most existing CRISPR/Cas12a methods for small molecules detection employ DNA aptamers as an intermediary, while RNA aptamers remain underexplored. The conventional CRISPR/Cas12 system offers limited programmability due to its partially conserved crRNA sequence. In this study, we developed an aptamer-regulated CRISPR/Ca...
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Aug 2026
A cascaded CHA-CRISPR/Cas12a photoelectrochemical platform enabled by S-scheme Bi2WO6/BiOBr for piR-823 detection.
Exosomal piR-823 is a promising specific biomarker for colorectal cancer, yet its ultrasensitive detection remains challenging due to its extremely low abundance and high sequence complexity. Herein, we developed a cascaded CHA-CRISPR/Cas12a photoelectrochemical (PEC) sensing platform enabled by an S-scheme Bi2WO6/BiOB...
Xia-Wen Lin, Peng Ye, Yangyang Ding et al.
· In Analysis · 0 citations
Aug 2026
CRISPR/Cas12a-based tag-free fluorescent biosensor using G-quadruplex specific thioflavin T for detection of oncogenic microRNAs.
Tag-free fluorescence biosensing platform for the detection of circulating miRs in serum, targeting microRNA-21 (miR-21) and microRNA-10b (miR-10b) as clinically relevant oncogenic markers, and demonstrates excellent sequence discrimination capability.
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Phosphorothioate-Enabled Cas12a Autocatalytic Network for Amplification-Free Ultrasensitive Nucleic Acid Detection.
Autocatalytic CRISPR/Cas systems offer a promising route toward amplification-free nucleic acid detection, yet their performance is limited by indiscriminate transcleavage of ssDNA activators and the reliance on structurally complex, thermodynamically unstable DNA architectures. A phosphorothioate (PS)-enabled Cas12a a...
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