2026· Methods in molecular biology· Vol 3018, pp.
85-98
· 0 citations
Medicine
TL;DR
Hydrophilic interaction chromatography is utilized to enrich glycopeptides from both peptides bound to HLA and digest of HLA proteins that obtained from immunoprecipitation of MHC complex from cell lysate that enables the characterization of glycosylation of MHC by database search.
The composite glycan population of each of the N-linked and O-linked glycosylation sites present on the Pentamer is determined by multienzymatic proteolysis and mass spectrometry, and the presence of underprocessed oligomannose-type glycans on some glycosylation sequons is revealed.
Antonio Lembo, Gian Luca Sardone, Michela Aurilia et al.· Glycobiology· 0 citations
HLA-Shuttle restores antigen presentation in immunologically cold tumor cells, facilitating identification of TAAs with favorable therapeutic potential and identifying multiple novel tumor associated antigens (TAAs) from both known and novel cancer immunotherapy targets.
Daniel Hwang, Molly C. Erdman, Santosh Adhikari et al.· Journal of Immunology· 0 citations
A novel method is presented for the expression in eukaryotic cells of secreted peptide exchange–competent class I MHC proteins in their native conformation and it is demonstrated that tetramers produced in this way are equivalent to conventionally produced tetramers for T-cell staining.
V. Ramachandiran, J. Shires, Richard A. Willis et al.· Journal of Immunology· 0 citations
The main mechanisms of antigen acquisition, endocytic/lysosomal factors controlling MHCII-restricted processing and presentation, and evidence linking lysosomal dysfunction to autoimmunity are explored.
Gabriele Sergio Colangelo, Kyra J Cowan, Federico Riccardi Sirtori et al.· Journal of Immunology· 0 citations
The results of this study indicate verifiable mechanisms of molecular action of alloferon, and suggest its role as an antigenic epitope, similar in structure to fragments of viral capsid proteins, facilitates the destruction of viral and bacterial pathogens.
I. Torshin, A. N. Gromov, O. Gromova· FARMAKOEKONOMIKA. Modern Pha...· 0 citations
A high-throughput assay to quantify stability of thousands of user-defined pHLA produced in E. coli and generated customizable pHLA stability datasets which show how peptide sequence motifs affect pHLA stability, and may be helpful for improving the authors' mechanistic understanding of pHLA stability.
M. Wilbrink, Luis O Correa-Medero, Emma C Duggan et al.· Journal of Immunology· 0 citations
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