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Jul 2026
High-cell-density fermentation of Komagataella phaffii enables the overproduction of a novel, broad-substrate endoglucanase, WcEG394 from Fuling (Wolfiporia cocos), for lignocellulose biorefining.
Recombinant WcEG394 efficiently hydrolyzed carboxymethyl cellulose, microcrystalline cellulose, and filter paper, and demonstrated broad substrate specificity toward xylans, pectin, chitin, agarose and agricultural residues such as corn cob and sugarcane bagasse.
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· International Journal of Bio... · 0 citations
Open access
Jul 2026
Tri-valorization of methanol in a single bioreactor: co-production of enzyme, chemical, and single-cell protein using engineered Pichia pastoris (Komagataella phaffii)
The economic viability of methanol-based biomanufacturing, particularly with green methanol as feedstock, is often limited by the low value of single-product processes. Here, we developed an integrated co-production strategy in the methylotrophic yeast Pichia pastoris (Komagataella phaffii) for the simultaneous convers...
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· Bioresources and Bioprocessi... · 0 citations
Open access
Jul 2026
Heterologous Expression of a Neurospora crassa Catalase Reprograms the Cellulase System and Enhances β-Glucosidase Production in Trichoderma reesei
Efficient saccharification of lignocellulose, the most abundant renewable carbon reservoir resource, is of great industrial importance. Trichoderma reesei is a premier cellulase producer, but its fermentation efficiency is often constrained by dual challenges: dissolved oxygen limitation and intrinsic oxidative stress....
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Open access
Aug 2026
Multistrategy metabolic engineering of Talaromyces pinophilus for α-amylase production from lignocellulosic biomass
Metabolic engineering of Talaromyces pinophilus through promoter optimization, multicopy integration, and protease deletion enables efficient α-amylase production from lignocellulosic biomass, achieving 26 712 U/mL in bioreactor fermentation.
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Jul 2026
High-density cultivation of Lacticaseibacillus paracasei GY-1 in a novel medium produces a structurally distinct immunostimulatory exopolysaccharide.
A novel multifunctional Spirulina platensis (SP) broth is developed, designated MSP, for cultivating Lacticaseibacillus paracasei GY-1, the first versatile medium that enables HCDC, enhances r-EPS yield, and produces structurally distinct r-EPS with potent immunostimulatory activity in a single batch culture, highlight...
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· International Journal of Bio... · 0 citations
Jul 2026
Characterization of a Novel GH3 β-Glucosidase with Alkali-Tolerant and Halophilic Properties from Pontixanthobacter luteolus SW-109.
Local enrichment of acidic residues on the PlGH3 surface could generate a negative electrostatic potential, which enables adaptation to high-salt and alkaline environments, thereby sustaining the enzyme's catalytic activity under such extreme conditions.
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