1st Order Derivative, AUC UV Spectrophotometry and Assay by RP-HPLC of Evogliptin tartrate in Tablet

Abstract

There are three new, modest, true and exact methods validated and developed on the criteria of ICH regulations for evaluation of Evogliptin tartrate in tablet and its pure form. Method 1: AUC by UV spectrophotometry technique utilizing 262 to 266nm for measurement of analyte in tablet. Method 2: 1st derivative spectrophotometry utilizing 258nm for estimation of analyte in the tablet. The range and linearity were found to be 270 to 10µg/ml for both methods. The standard and sample solutions were prepared from methanol. Less than 2 was found less than 2 and recovery study of both Method. Method 3 consists RP-HPLC method using enable C18 column and containing Water and Methanol mobile phase in the ratio of 30 and 70. The 1ml/min of flow rate, with detection at 267nm, and temperature 250C. 2.806min was the retention time found. 10-80µg/ml was the linearity range which found. Y = 4006.6714 x + 713.6349 was the linear regression equation and Correlation coefficient (r2) = 0.9999. Less than 2 is the %RSD in the robustness, reclamation and precision investigations of the programmed method. Reported method was robust, accurate and precise in nature. Method-3 is more accurate and precise in comparison to method 1 and 2.