Dual-readout lateral flow immunoassay based on upconversion nanoparticle-gold nanoparticle luminescence resonance energy transfer for rapid determination of 17β-estradiol
A luminescence resonance energy transfer (LRET)-regulated dual-readout lateral flow immunoassay (LFIA) is reported that integrates naked-eye colorimetric screening and near-infrared-excited quantitative luminescence analysis on a single test strip, offering a versatile platform for rapid on-site screening of small-molecule contaminants in complex matrices.
The rapid, sensitive, and quantitative detection of small-molecule analytes in complex matrices remains fundamentally constrained by severe optical interference and limited signal transduction efficiency. Herein, we report a turn-on near-infrared (NIR) fluorescence lateral flow immunoassay (NFLFIA) that integrates indo...
Jing-Rui Yuan, Liang Huang, Ya-Qi Luo et al.· Analytical Chemistry· 0 citations
A dual-mode colorimetric–photothermal LFIA platform was successfully constructed for the detection of thrombosis-related biomarkers, including thrombomodulin and α2-plasmin inhibitor–plasmin complex, in clinical serum samples, enhancing analytical sensitivity and reliability.
Jun Yin, Yu Qiu, Yao Nie et al.· RSC Advances· 0 citations
A dual-aptamer-based, dual-mode FRET lateral flow strip is developed for the rapid and sensitive detection of thrombin in serum samples, achieving a fluorescence-based detection limit of 0.135 nM-twice as sensitive as the colorimetric method.
A preliminary evaluation using serum samples from five breast cancer patients and five healthy individuals showed clearly distinguishable colorimetric and fluorescent responses between the two groups, supporting the effectiveness of the proposed probe for miRNA-21 detection in real serum samples and indicating the pote...
Ruien Shi, Qian Xiang, Yajuan Chang et al.· Talanta: The International J...· 0 citations
BACKGROUND
Foodborne pathogens remain a major threat to public health and food safety, among which Salmonella typhimurium is a leading cause of bacterial foodborne illness. Conventional culture-, immunoassay-, and nucleic-acid-based methods are reliable but often require laboratory facilities, trained personnel, and re...
Lun Luo, Renjie Zhou, Wenhai Wang et al.· Analytica Chimica Acta· 0 citations
D-Proline has been reported as a potential biomarker for gastric (stomach) cancer, creating a demand for practical and rapid analytical approaches for early diagnosis using simple and non-invasive clinical samples. In this work, a microfluidic paper-based analytical device (µPAD) integrating molecularly imprinted polym...
Akarapong Prakobkij, Jiajia Zhou, Mahnaz Maddahfar et al.· In Analysis· 0 citations
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