Jul 2026· Food Science and Biotechnology· Vol 35, pp. 3349 - 3359· 0 citations· 36 references
Medicine
Abstract
The study re-analyzed the draft genome of Bacillus licheniformis SMIA-2 and generated a reference-guided pseudo-scaffold. Cross-validated genome annotation identified five candidate loci associated with pectin degradation, including putative pectate lyases, polygalacturonase, and downstream uronate-catabolic genes. Submerged fermentation with passion fruit peel flour and corn steep liquor yielded crude enzymatic extracts, which were spray-dried at 110 °C using maltodextrin and microcrystalline cellulose as stabilizers. The dried formulation retained pectinase activity for 180 days at 5 °C and showed additional cellulase, amylase, xylanase, and protease activities. Pectinase displayed optimal activity at pH 8.5 and 70 °C, with stability between pH 8.0–8.5 and 65–70 °C. Despite not using a reference strain and the absence of some omics analyses, with genomic and industrial claims presented as evidence of biotechnological potential rather than definitive functional validation of individual genes, these results support a sustainable, scalable, and alkaline-tolerant enzyme platform based on agro-industrial residues.
The results highlight the potential of thermo-alkali stable polygalacturonase from extremophilic actinomycetes as a long-term and cost-effective biocatalyst for agro-waste valorization and industrial fruit juice processing.
A. E. Gomaa, Mohamed H. El-Sayed, O. Atta et al.· Biodegradation· 0 citations
Cocoa bean quality is strongly influenced by microbial fermentation, which drives flavour development through enzymatic activity. Despite its importance, the specific microorganisms, particularly those producing pectinase, remain poorly characterized. This study aimed to identify and evaluate pectinase-producing microb...
Angela Guma Berwin, I. Amoako-Attah, S. Opoku et al.· Applied microbiology· 0 citations
BSEG2 is highly thermostable, tolerant of high salt and ionic liquids, and notably resistant to cellobiose inhibition up to 200 mM, suggesting potential to reduce freshwater demand in biomass processing and a strong candidate for simplified, cost-effective enzyme formulations for glucose production from lignocellulosic...
Debjyoti Ghosh, Aditi Konar, Yi Gao et al.· Biotechnology Journal· 0 citations
Production and characterize of β-glucosidases from the crude extract of the cyanobacterium Microcystis aeruginosa CACIAM 03 reinforce the promising enzymatic potential of M. aeruginosa strains isolated from the Amazon region for sustainable biotechnological applications.
G. Serra, Matheus Silva da Fonseca Diniz, E. Gonçalves et al.· Biotechnology and applied bi...· 0 citations
Local enrichment of acidic residues on the PlGH3 surface could generate a negative electrostatic potential, which enables adaptation to high-salt and alkaline environments, thereby sustaining the enzyme's catalytic activity under such extreme conditions.
Kaijuan Wu, Ke Guo, Zheng Yu et al.· Applied Biochemistry and Bio...· 0 citations
